Odontogenic Fascial Infections Involving Primary Molars Affected with S-ECC in Indian Children: Evaluation of Microbiota, Antibiotic Sensitivity & Antibiotic Resistance via Vitek 2 System

Shaik Hasanuddin1, J Sharada Reddy1, Jyothi Lakshmi2, S Pavani2, Mohammed Abdul Mohi Siddiqui3
1 Department of Pedodontics & Preventive Dentistry, Government Dental College & Hospital, Hyderabad, Hyderabad, Telangana, India
2 Department of Microbiology, Osmania Medical College, Hyderabad, Telangana, India
3 Department of Microbiology, Osmania General Hospital, Hyderabad, Telangana, India

Background :

The aims and objectives of the study are :

  • To evaluate the microbiota from the aspirate obtained from odontogenic fascial abscesses involving primary molars in children affected with ECC-S type in the age group of 4-12 years, over a period of 24-36 months.
  • To evaluate antibiotic resistance in pure cultures of microorganisms via the Vitek 2 system.

Methods :

Pediatric patients in the age group of 4-12 years, suffering with odontogenic fascial space abscess were selected in the study sample. The sample size was limited to around 100 patients annually and a total sample size of 300-400 patients for the entire study period shall be considered. The selected patients were grouped into two Groups :

Group 1 : Patients who are suffering with first time odontogenic infection.

Group 2 : Patients who are suffering with a second or third episode of an odontogenic space infection with the same region/tooth or a different region/tooth.

An aspirate via a 22 Guage wide-bore needle for culture was collected intraorally, for obtaining pure culture of aerobic and anaerobic microbiota. Pure cultures were subjected to antibiotic sensitivity and resistance testing via VITEK-2 Compact system.

Results :

The data thus obtained from the diagnostic test (Vitek 2 software) shall be recorded and subjected to statistical analysis. The most common organism causing fascial abscess in children suffering with ECC-S type can be identified. The percentage of children with antibiotic resistance to commonly used antibiotics can be identified.

Conclusion : .

Streptococcus pyogens is the predominant microorganism found in pus cultures.

Public health policy regarding the management of ECC, should include the precise use of antibiotics in children, especially those suffering with systemic diseases, disabilities and special children.

In severe cases, use of Vitek 2 compact system to check antibiotic resistance should be advocated.

Hasanuddin Shaik