A New Method to Analyze Heart Culture Arrhythmia



Avinoam Rabinovitch1, Yaacov Biton1, Doron Braunstein3, Menahem Friedman4, Ira Aviram1, Omer Berenfeld2, S.V. Pandit2
1 Physics, Ben-Gurion University, Israel
2 Center for Arrhythmia Research, University of Michigan, USA
3 Physics, Sami Shamoon College of Engineering, Israel
4 Information Systems Engineering, Ben-Gurion University, Israel

Aims: To highlight the inherent patterns and study the question of how a fibrillating heart can be driven and operated by a single source, notably a spiral.

Methods and Results: Singular value decomposition (SVD) analysis was applied to an experimental model of fluorescence-based mapping of cardiac fibrillation in cells culture.

We demonstrate how usage of the SVD method is able to demarcate regions harboring drivers (spirals, having high “ranking”) and regions of influenced activity (with low ranking). The analysis further enhances the wave breaking in the transfer region (See Fig.1).

A Granger causality analysis (GCA) is applied to the leading normal modes of the SVD analysis and its results demonstrate that indeed the spiral modes are the dominant ones, driving the modes into other parts of the culture.

Conclusions: These results are of importance for better understanding of the dynamics of excitation in heart fibrillation. They demonstrate the importance of a driving rotor, rather than the assumption of ectopic target wave source, as the leading drivers of fibrillation. Note that, in the original videos prior to the SVD-GCA analysis, it is impossible to discern between a rotor and an ectopic source.